For Researchers

A2CPS Study Details

To learn more about the Acute to Chronic Pain Signatures study, explore the About section, including:

Study Protocol
Biomarkers we’re measuring
Psychophysical Assessments we’re making
Omics from Blood
Brain Imaging data we’re collecting
About the Consortium.

For more detail, see our published Biomarkers paper in PAIN and our Protocol paper published in Frontiers in Medicine.

A2CPS Internal Projects

Project Title	Lead/Senior Project Author	QST	EHR	Patient Reports	Functional Testing	Omics	Imaging
Polygenic score for chronic pain and its correlations with experimental pain, related psychosocial phenotypes, and biomarker levels.	Katerina Zorina-Lichtenwalter	✓	✓	✓		✓
Statistical models of imaging of pain	Enamul Bhuiyan			✓			✓
Mediating effect of movement-evoked pain on the relation between pain interference and physical function	Giovanni Berardi	✓	✓	✓	✓
Differences and Similarities in Biomarkers and Other Measures between Surgical Cohorts	Briha Ansari	✓		✓		✓	✓
Multivariate psychosocial predictive model for chronic pain: Correlations with pain intensity	Michael Sun	✓	✓	✓	✓	✓	✓
Brain signatures of widespread pain and sensory sensitivity	Rick Harris / Chelsea Kaplan	✓		✓			✓
WGCNA Correlation of molecular, imaging, and psychosocial measures in the TKA cohort at baseline	Michael Olivier	✓		✓	✓	✓	✓
Alignment of EHR and self-reported medication information with mass spectral data	Michael Olivier		✓			✓	✓
Characterizing racial/ethnic representation within A2CPS	Chad Brummett / Rachel Bergmans
Correlation of obesity-related proteins with Body Mass Index in A2CPS baseline samples	Panshak Dakup / Jon Jacobs			✓		✓
Brain age, estradiol; women's health; sex differences	Patrick Sadil / Tanja Jovanovic / Lara Zador		✓	✓		✓	✓
Exploring Immune Transcriptome Signatures in the Development of Chronic Pain	Margaret Taub / Kristen Jepsen			✓
Prevalence of and Association of Risk Factors with Unhealthy Substance Use Before Surgery	Mark Bicket			✓
Multi-omics correlates of fatigue in baseline data from the knee osteoarthritis cohort	Giovanni Berardi	✓		✓	✓	✓
Exploring the relationship between fatigue and measures of altered central nervous system signal processing.	Adam Janowski	✓	✓	✓	✓		✓
Examining the effects of the Melanocortin-1 receptor (MC1R) gene on clinical pain, pain sensitivity, opioid, substance use, psychosocial status, and brain imaging	Ezgi Genc	✓		✓		✓	✓
Impact of resilience on risk factors for chronic pain at presentation for total knee arthroplasty.	Bob McCarthy	✓	✓	✓	✓	✓	✓
Machine Learning Models to Classify Hypertensive Patients Through Diverse, Extensive Multimodal Biomedical Data	Seth Berke		✓	✓	✓	✓	✓
Normalization of Luminex Proteomics	Seth Berke					✓
Pain Sensitivity- Pain and Pain Sensitivity Assessments in the Acute to Chronic Pain Signatures (A2CPS) Program	Laura Frey-Law	✓		✓	✓
Omics- Multi-omic data generation in the Acute to Chronic Pain Signatures (A2CPS) project	Margaret Taub					✓
EHR Data- Electronic Health Records Acquisition and Processing in the Acute to Chronic Pain Signatures (A2CPS) Study	Briha Ansari / Robert McCarthy		✓	✓
Psychosocial	Emine Bayman			✓
Major Outstanding Questions in Pain Science	Stephani Sutherland
Pivoting during the Pandemic: Virtual Training and Certification of Multi-site Research Staff	Carol Vance	✓			✓	✓	✓
A Beta-Binomial Model for Analyzing Zero- or One-Inflated Pain Trajectories	Nora Liu			✓
Image Processing in the Acute to Chronic Pain Signatures (A2CPS) Project	Patrick Sadil			✓			✓
Replication of Fibromyalgia Score (WPI+SSI) GWAS Signals in A2CPS and Meta-analysis with MGI	Stephan Frangakis			✓
Multi-order neural correlates of pain	Li-Bo Zhang	✓	✓	✓	✓		✓
Functional and structural brain predictors of peripheral immunity and pain sensitivity	Byeol Kim Lux	✓	✓	✓	✓	✓	✓

A2CPS Primary Biomarkers

Patient Reported Outcomes & Behavior

Primary Biomarkers	Assessment
1. General pain intensity	Brief Pain Intensity -whole body pain
2. Local pain intensity	Modified BPI – surgical site pain
3. Widespread body pain	Michigan Body Map
4. Acute pain trajectory following surgery	Single item assessments of daily pain and/or pain interference during post-surgical period.
5. Disability	Knee Injury and Osteoarthritis Outcome Score – 12 (TKA only) Danish Thoracic Surgery Questionnaire (Thoracic surgery only)
6. Physical function	PROMIS Short Form v2.0 Physical Function 8b Five Times Sit-to-Stand, 10 Meter Walk Test (TKA only)
7. Movement-evoked pain	Five Times Sit-to-Stand, 10 Meter Walk Test (TKA only) Coughing and deep breathing (Thoracic surgery only)
8. Anxiety	General Anxiety Disorder – 7
9. Depressive symptoms	Patient Health Questionnaire – 8
10. Pain catastrophizing	Pain Catastrophizing Scale – 6
11. Fear of movement	Fear Avoidance Beliefs Questionnaire – Physical Activity
12. Sleep	PROMIS Short Form v1.0 Sleep Disturbance 6a Sleep duration = time of sleep obtained over the past month
13. Trauma history	Adverse Childhood Experience questionnaire
14. Resilience	Pain Resilience Scale
15. Social support	PROMIS SFv2.0 Instrumental Support 6a PROMIS SFv2.0 Emotional Support 6a
16. Cognitive dysfunction	Multidimensional Inventory of Subjective Cognitive Impairment

Omics

Primary Biomarkers	Assessment
17. C-reactive protein	Proteomics/Luminex
18. Inflammatory markers – Tumor necrosis factor-alpha	Proteomics/Luminex
19. Inflammatory markers – Interleukin-6	Proteomics/Luminex
20. Inflammatory markers – Interleukin-12	Proteomics/Luminex
21. Soluble glycoprotein 130	Proteomics/Luminex
22. Catechol-O-methyltransferase haplotype (rs4680)	Genotyping Array
23. Mu-opioid receptor (rs1799971)	Genotyping Array
24. ATP binding cassette subfamily B1 (rs1045642)	Genotyping Array
25. Brain derived neurotrophic factor (rs6265)	Genotyping Array
26. Brain derived neurotrophic factor (rs1491850)	Genotyping Array

Quantitative Sensory Testing (QST)

Primary Biomarkers	Assessment
27. Pressure pain threshold (PPT)	Pressure algometer at surgical site
28. Temporal summation (TS)	Punctate stimulus (Neuropen®) at surgical site
29. Conditioned pain modulation (CPM)	Change in shoulder PPT following noxious cold water submersion

Brain Imaging

Primary Biomarkers	Assessment
30. Gray matter volume of medial prefrontal cortex	T1
31. Structural integrity in Am-NAc-mPFC network	Diffusion weighted imaging, white matter tractography
32. Core DMN vmPFC/CCC–NAc/ventral striatum	rsfMRI
33. Core DMN: vmPFC/CCC–somatosensory (dplNS/S1)	rsfMRI
34. Core DMN: vmPFC/CCC-anterior/middle insula	rsfMRI
35. Hub disruption	rsfMRI
36. Evoked response in neurologic pain signature	Task fMRI (pressure cuff)
37. Evoked response in fronto-striatal systems related to descending / central pain modulation and self-regulation (vmPFC, NAc)	Optimized markers (SIIPS)